Survey Antibiogram Pattern in ESBL Producer Klebsiella pneumoniae Strains Isolated of Urinary Tract Infection
Shilla Jalalpoor Teacher of Microbiology,
Islamic Azad University Shahreza Branch,
Membership of Young Researchers Club.
Islamic Republic of Iran
Abstract:
Introduction and Objectives
ESBLs are defined as β-lactamase capable of hydrolyzing third generation cephalosporin's and inhibited by β-lactamase inhibitor.Some of the species of gram negative bacilli including Klebsiella pneumoniae are known to be producing among Bacteria. Klebsiella pneumoniae are a relevant opportunistic pathogen that accounts for nosocomial infections. Antibiotic resistance in ESBLs strains is one of the emerging health related problem in the world nowadays.
Materials and Methods: The search were laboratory and performed in Azzahra and Shariaty hospitals in 2009 year in Isfahan, according to statistical formula randomly selected 91 samples from urinary infections.Bacterial identification was performed with microbiological methods. ESBLs determine were in two section, screening and confirming testing were respectively included by Kirby-Bauer's disc diffusion and Combining disc methods and antibiogram was performed with Kirby-Bauer's method.
Results:
From 91 samples frequency of Klebsiella pneumoniae strains was 15/4% (57.1% from out patients and 42.9% from hospitalized patients).From 14 isolated Klebsiella pneumoniae strains, 35.71% product ESBL. According to antibiogram result respectively 100%, 100%, 60%, 60%, 60%, 40%, 0% and 0% of ESBL producer Klebsiella pneumoniae strains were resistant into, Ceftazidime, Ampicillin, Ceftazidime, Co-Trimoxazole, Amikacin, Nitrofurantoin, Ciprofloxacin and Imipenem.
Conclusion: According to result antibiotic resistant and ESBLs production were higher in hospitalized patient. These because cephalosporin's are usually first line against to many severely infections disease. Justifiable use of will be an effective means of controlling and decreasing spread of ESBLs strains.
